Coding

Part:BBa_K531006:Design

Designed by: Qimeng Gao   Group: iGEM11_Grinnell   (2011-07-26)


Optimized esp gene for C. crescentus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA codons are optimized for C. crescentus expression.


Source

Wild type esp sequence from Zhang,Y.Q., Ren,S.X., Li,H.L., Wang,Y.X., Fu,G., Yang,J., Qin,Z.Q., Miao,Y.G., Wang,W.Y., Chen,R.S., Shen,Y., Chen,Z., Yuan,Z.H., Zhao,G.P., Qu,D., Danchin,A. and Wen,Y.M. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49 (6), 1577-1593 (2003). Optimized sequence is based on the wild type sequence.

References